Blar i forfatter "Klitgaard, Mette"
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Mimicking regional and fasted/fed state conditions in the intestine with the mucus-PVPA in vitro model: The impact of pH and simulated intestinal fluids on drug permeability
Falavigna, Margherita; Klitgaard, Mette; Steene, Erik; Flaten, Gøril Eide (Journal article; Tidsskriftartikkel; Peer reviewed, 2019-02-27)Intestinal drug absorption following oral administration can be influenced by regional conditions (absorbing surface area, bacterial flora, motility, pH, mucus thickness) and food intake, all of which affect drug solubility and permeability. Therefore, it is crucial to assess the impact of these conditions on the drugability of drugs and formulations. In this study, the ability of the liposome-based ... -
Mucus-PVPA (mucus Phospholipid Vesicle-based Permeation Assay): An artificial permeability tool for drug screening and formulation development
Falavigna, Margherita; Klitgaard, Mette; Brase, Christina; Ternullo, Selenia; Skalko-Basnet, Natasa; Flaten, Gøril Eide (Journal article; Tidsskriftartikkel; Peer reviewed, 2017-12-26)The mucus layer covering all mucosal surfaces in our body is the first barrier encountered by drugs before their potential absorption through epithelial tissues, and could thus affect the drugs’ permeability and their effectiveness. Therefore, it is of key importance to have in vitro permeability models that can mimic this specific environment. For this purpose, the novel mucus phospholipid vesicle-based ... -
Predicting oral absorption of fenofibrate in lipid-based drug delivery systems by combining in vitro lipolysis with the mucus-PVPA permeability model
Falavigna, Margherita; Klitgaard, Mette; Berthelsen, Ragna; Mullertz, Anette; Flaten, Gøril Eide (Journal article; Tidsskriftartikkel; Peer reviewed, 2020-09-08)The aim of this work was to develop a new in vitro lipolysis-permeation model to predict the in vivo absorption of fenofibrate in self-nanoemulsifying drug delivery systems (SNEDDSs). More specifically, the in vitro intestinal lipolysis model was combined with the mucus-PVPA (Phospholipid Vesicle-based Permeation Assay) in vitro permeability model. Biosimilar mucus (BM) was added to the surface of ... -
Simultaneous assessment of in vitro lipolysis and permeation in the mucus-PVPA model to predict oral absorption of a poorly water soluble drug in SNEDDSs
Falavigna, Margherita; Brurok, Sunniva; Klitgaard, Mette; Flaten, Gøril Eide (Journal article; Tidsskriftartikkel; Peer reviewed, 2021-01-21)The prediction of the <i>in vivo</i> performance of self-nanoemulsifying drug delivery systems (SNEDDSs) is currently gaining increasing attention. Therefore, the need for reliable <i>in vitro</i> models able to assess the drug solubilization capacity of such formulations upon <i>in vitro</i> lipolysis, as well as to concomitantly evaluate <i>in vitro</i> drug permeation, has become ever so evident. ...